Uji Aktivitas Anti Bakteri dari Fraksi Daun Areuy Kikunti (Pothos Junghuhnii De Vreise) terhadap Bakteri Staphylococcus Aureus dan Escherichia Coli

Abstract

Traditional medicinal plants are popular plants to be used as the raw material of traditional medicine, for example, Pothos leaves (Pothos junghuhnii de vriese). This study aimed at observing the N-Hexane fraction, ethyl acetate, and butanol from Pothos leaves (Pothos junghuhnii de vriese) that can inhibit the bacteria growth in Staphylococcus aureus and Escherichia coli by using the disk diffusion method. The solvents used for the fraction of Pothos leaves were N-hexane, ethyl acetate, and butanol. The test solvent used in antibacterial testing was the solvent for fractionation at various concentrations, namely 31.25 μg/mL, 62.5 μg/mL, 125 μg/mL, 250 μg/mL, and 500 μg/mL). Moreover, an antibiotic disk, namely chloramphenicol, was a positive control, while DMSO was the negative control. The result showed that the fraction of Pothos leaves had antibacterial activity against Staphylococcus aureus and Escherichia coli. The fraction of ethyl acetate with a diameter of 10.5 mm and Escherichia coli with a diameter of 8.5 mm at a concentration of 500ug/ml were in the ‘poor’ category